Fax 4200 BERTHOLD TECHNOLOGIES Lumat LB 9507 Tecan SpectraFluor Thermo Scientific Varioskan LUX LJL Biosystems Analyst AD 96. of 0. Growth medium was then replaced with fatty acid uptake solution. . 3. Integrated stacker option. 5% at 1. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Running both standard spectrophotometer and microplate. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. ± 0. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. These instruments are all elegant and simple in their form and function. This is equivalent to having 511 built-in filters,Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. The Multiskan Sky microplate reader is an absorbance plate reader. Keywords: fluorescence polarization, trf, fret, igg. Documentation:1. Can be integrated with Molecular Devices' StakMax® Microplate Stacker. Supplementary Information. 384-well: 0. 54: 1. 300 µL of Detergent Compatible Bradford reagent was added to each well. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesThe SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. A. The Molecular Devices Versamax Microplate Reader INCLUDED:. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. 96- or 384-well microplate compatibility. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. ValitaTiter Assays Setup Guide 2601A October 2023. #N207) LAL Reagent Water (Lonza cat. Microplates . measurements in compatible Molecular Devices® microplate readers. Contents of wells were pipetted up and down several times to mix. SpectraMax Microplate Readers. Click on the crosshair and move it so that it is centered over the well image. 100 fmol 1536-well (8 µL) 0. We would like to show you a description here but the site won’t allow us. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. The device was able to achieve 3σ limits of detection of ca. Align the two slots on the bottle holder with the two pegs on the rail. Less background noise gives a better limit of detection and so a relative increase in usable signal. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. 2. Wavelength Range 200 to 999 nm. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. Deep well blocks available in 24, 96 and 384-well formats Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 96-well-plate-scan-time-2-colors-1-site-well. reagent basin for ease of pipetting into. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. Compact design Minimum volume and. We have been using this for DNA measurements 260/280 nm, colorimetric assay measurements like MTT assays, and ELISAs. Or scan up to 384 wells at any time to determine the best assay conditions. All stand-alone instrument functions can be controlled using the software. In . The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). adjust the reader. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Molecular Devices SpectraMax 384 Plus Microplate Reader. . #L6876) • Guanidine hydrochloride (Sigma cat. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. Description. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. When reading at wavelengths. Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. plate height between 12–16 mm and 96-well “strip” plates Barcode libraries Interleaved 2 of 5, Code 128, EAN 128, Code. All stand-alone instrument functions can be controlled using the software. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aThe Molecular Devices Aquamax 4000 With 96 Well Head AQUAMAX 4096-G is a quality addition to the Molecular Devices lineup. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. #1402-9700) 384-well low volume solid black microplate (Corning cat. Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Total volume (except deep. 00 Add to cart; Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. 92: 88. microplate reader (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc manufactures this product . #12-639) White 96- and 384-well microplates (Greiner cat. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. Listing: #4772697. Or scan up to 384 wells at any time to determine the best assay conditions. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. SOP Title: Use and. Whatever your needs, you’ll find a microplate reader that can meet them. While they play an important role in generating data from a wide range of. for 96- and 384-well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools SpectraMax® L Microplate . The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Solid black plates usually give the least fluorescent background noise, allowing better sensitivity. In addition, the iD5 reader measures TRF and FP and. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. microplates or in a cuvette at a. book Page 1 Wednesday, October 12, 2011 4:56 PMBuilt around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. explore more. Plate was read with SpectraMax 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) using the. 03 pM 384-well: 0. 39. Open the catalog to page 5. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Suggested plates are black walled. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Endpoint, kinetic, spectrum and area-well scanning Click here. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. With optimized reagents, option to operate the. SpectraMax® M3/M4/M5/M5e, GeminiTM EM/XPS, FlexStation® 3. 125 pM * Specifications listed are for europium 96-well: 0. 0 OD at. Microplate Readers. personalized as your research. ValitaTiter 96-well (Pack of 20). The SpectraDrop Microplate’s slide design eliminates the need for calibration and gives consistent well-to-well reads with CVs below 5%. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. ©2010 molecular Devices, inc. 05. iD5. Read one sample or up to 384 in a. 6:30 . Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. Multimode Readers Comparison Chart. 2. Published Dec 16, 2020 | Updated Dec 16, 2020. 300 µL of Detergent Compatible Bradford reagent was added to each well. Three kits covering a wide range of dsDNA concentrations. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. The iMark. com. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Microplate Reader, Molecular Devices Optimax, Refurbished. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. This reader's dual monochromators for. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. 585The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. I contacted Molecular Devices and they quoted me almost $6k to come out and replace. Monochromatic filter is equipped. Microplate Capacity Standard 96-well microplates Incubation Temperature control up to 50ºC Temperature uniformity ± 0. Never order or replace filters to optimize an assay again!microplate readers from Molecular Devices. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. LVF Monochromator™ : Excitation and Emission. Four wash/rinse bottles are included and configurable for both 96- and 384. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. Microplate method: Pipet 150 µL aliquots of each sample, standard and blank into the designated wells of the microplate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Condition: Used - Good. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. #I2511) • Assay diluent – may be cell culture medium. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. softmaxt pro microplate data analysis, molecular devices’ stakmax microplate stacker, monochromator-based fluorimeter, wavelength optimization, fda 21 cfr part 11. Microplate Spectrophotometers. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Step 2: Launch SoftMax Pro Software. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit (signal 3X SD of baseline) 8. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. Fluorescence Microplate Reader Comparison Chart. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Measure more accurately. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. " A versatile UV/VIS spectrophotometer for both 96- and 384-well microplates as well as cuvette. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all leading partner robots. The reader adds up to three transfers of assay reagents from a 96- or 384. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. The EMax® Plus Microplate Reader is designed to be a versatile and robust microplate reader for research laboratories. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. #3610) BrightMax sealing films (Genesee cat. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. *Simple analysis and optimization. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Volume is not critical because water does not absorb light appreciably between 190 and 900 nm. It also allows for the elimination of standard curves. Key applications: ELISA, nucleic acid, endotoxin testing. Vrla used the device to quantify the concentration of a small molecule produced by. Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. Avoid losing high signal. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 2022 Harini Natarajan et al. The VersaMax reads 96-well microplates. Fluorescence Microplate Readers. 1. #I2511)Introduction. 4. absorbance, fluorescence, and luminescence with the added functionality of. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 USA Tel: 408. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPlus Microplate Reader, the R2 value was 0. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Published Dec 16, 2020 | Updated Dec 16, 2020. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). 92: 88. Model:. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. 747. # 651101) the iD5 reader’s filter system. Plate Formats: 6 to 384-well microplates. Lucid Scientific. July 17, 2023. 96-well flat clear bottom white TC-treated microplates (Corning cat. Optical System: High energy xenon flash lamp. Fluorescence Microplate Reader Comparison Chart. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. A microplate reader as . Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 1 OD at 230 nm. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Molecular Devices' microplate readers. microplate . maximum-illumination-channels. $489. Application Area: Spectrophotometer for molecular biology. Seller assumes all responsibility for this listing. 012 fmol 0. This page contains the. Microplate Reader (Molecular Devices cat. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. To confirm the accurate positioning of arrayed colonies, we performed an absorbance scan in a 96-well scheme with 30 × 30 scans per well for an OmniTray agar plate with 96. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). (Molecular Devices cat. BSA concentration (µg/mL) Average OD. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Eight filter modes enable applications such as protein quantification, cell viability and ELISA. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. 0 500 1000 1500 2000 2500 3000 3500 time (sec. Detection modalities are shown in Table 1-1. 4 Pages. Description. It also integrates with the. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. With various on-board features and capabilities,. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. Table 1: Categories of heating element design. Molecular Devices VersaMax Microplate Reader Specifications. #3676). changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. In . SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. Detection by Low noise PMT with simultaneous digital and analog modes. A single-mode plate reader is a good starting point. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Microplate readers use a vertical light path so the distance of the. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 0200-6263. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 7°C (gradient heating design). I have used Molecular Devices’ Vmax 96-well microplate readers since about the time of their inception in the mid to late-1980s. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Instrument settings can beAbsorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. With various on-board features and capabilities, the ThermoMax is. emax and Vmax microplate readers. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. +49-89/96-05-88-0 Fax +49-89/9-62-02-34-5 Japan. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. Endpoint, kinetic, spectrum. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. #ID3-STD) • L-Tryptophan (Sigma cat. The kinetic top-read mode of a SpectraMax M3 microplate reader (Molecular Devices, CA, USA). 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. The assay kit is optimized for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. g. Learn more about how absorbance is measured, and some key applications that utilize. Reading Capabilities: cuvette or microplate. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. These models can no longer be repaired. Use the provided 3. With various on-board features and. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. #W50-100). Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Triplicates of each BSA concentration were made for this assay plate. ZERO BIAS - scores, article reviews, protocol conditions and moreThe reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. (FP) configured microplate readers. #N207) LAL Reagent Water (Lonza cat. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Learn more 0200-7008 6- to 1536-well plates. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. flexibility of monochromator-based systems. The EMax Plus reader measures flat and round 96-well microplates. Detergent Compatible Bradford assay. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. 48 W x 12. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 100 fmol 384-well (75 µL) 0. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 1. Fluorescent DNA quantitation. ***Figure 1. iD5. ” Promega Corp. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Elisa Plate Readers and Microplate Readers at Biocompare. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SoftMax Pro Software allows you to set up and run a complete protocol for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Easy-to-use, intuitive, configurable microplate readers with industry-leading, SoftMax Pro microplate software solution for increased productivity. 1 sec. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. Outer wells are aligned for proper viewing with FLIPR. #SpectraMax L Config) Methods. Samples were run in triplicate. Microplate is validated by molecular devices for the FLIPR reader. If the best wavelengths are unknown, the Gemini EM reader will find. 05. Softmax Pro GxP Software. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Outer wells are aligned for proper viewing with FLIPR. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. Three low volume 24-well bottom slides. Infinite 200 Pro Microplate Reader. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Minneapolis, MN, USA), according to the manufacturer's protocol. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. If read values are low, try setting PMT sensitivity to High in the Settings menu of the Plate section. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Materials. 96-well solid bottom, clear polystyrene microplates (Greiner cat. 3.